《植物生理学报》 2018, 54(6): 1095-1102

高羊茅春化基因FeVRN2的克隆及其在非生物胁迫下的表达

陈莹, 陈锡, 蔡一鸣, 吴佳海, 杨义成, 王小利^*

贵州省草业研究所, 贵阳550006

通信作者：王小利；E-mail: wangxiaolizhenyuan@126.com

摘　要：

VRN2基因是受春化作用负调控的开花抑制子。为揭示非生物胁迫下高羊茅(Festuca elata)春化基因VRN2的分子调控机制, 本研究以高羊茅为实验材料, 采用cDNA末端快速扩增技术, 克隆得到VRN2基因全长cDNA序列, 命名为FeVRN2。FeVRN2基因cDNA全长为1 219 bp, 具有一个完整的长度为657 bp的开放阅读框, 编码蛋白质产物长度为218个氨基酸, 并包含一个CCT保守结构域。同源性分析表明FeVRN2与禾本科植物圆锥小麦(Triticum turgidum)、节节麦(Aegilops tauschii)、山羊草(Aegilops speltoides)、二穗短柄草(Brachypodium distachyon)、大麦(Hordeum vulgare subsp. vulgare)的亲缘关系非常近。荧光定量PCR结果显示: FeVRN2基因在高羊茅叶片中的表达受高温、干旱与高盐胁迫特异诱导, 说明该基因参与了高羊茅对高温、干旱与高盐胁迫的适应性调控。

关键词：高羊茅; FeVRN2基因; 克隆; 非生物胁迫

收稿：2017-12-22   修定：2018-04-25

资助：国家自然科学基金(31360576)和贵州省科学技术厅-贵州省农业科学院科学技术联合基金(黔科合LH字[2014]7699)。

Cloning and expression analysis of vernalization gene FeVRN2 in tall fescue under abiotic stress conditions

CHEN Ying, CHEN Xi, CAI Yi-Ming, WU Jia-Hai, YANG Yi-Chen, WANG Xiao-Li^*

Guizhou Institute of Prataculture, Guiyang 550006, China

Corresponding author: WANG Xiao-Li; E-mail: wangxiaolizhenyuan@126.com

Abstract:

VRN2 gene is a flowering repressor down-regulated by vernalization. To explore the molecular mechanism of VRN2 gene in tall fescue (Festuca elata) under abiotic stress, with mRNA from leaves of tall fescue as the template, VRN2 was cloned through rapid amplification of cDNA ends technique from the leaves of tall fescue and designated as F. elata VRN2 (FeVRN2). The whole sequence of FeVRN2 is 1 219 bp, with a 657 bp open reading frame (ORF), encoding a polypeptide of 218 amino acids. Domain analysis reveals that proteins encoded by VRN2 contain conserved domains (CCT). Phylogenetic analysis showed that FeVRN2 is closely related to other Gramineae plants such as Triticum turgidum, Aegilops tauschii, A. speltoides, Brachypodium distachyon and Hordeum vulgare subsp. vulgare. Fluorescence quantitative PCR results showed that expressions of FeVRN2 genes in tall fescue were distinctly induced under heat, drought and salt stresses, suggesting that FeVRN2 participates in heat, drought and salt stresses regulation in tall fescue.

Key words: tall fescue; FeVRN2 gene; cloning; abiotic stress